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ANTIBODY INFORMATION
 
 

Antibody HPA031188

  

Antibody HPA031189

  

Antibody HPA031190

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA031188   HPA031189   HPA031190  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 6   6   6  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA031188

  

Antibody HPA031189

  

Antibody HPA031190

  
Immunocytochemistry

Supported  

Validated
  

Validated
  
Immunohistochemistry


Validated
  

Validated
    
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031188

  

Antibody HPA031189

  

Antibody HPA031190

  

Formal validation: Independent

  
Validated

Antibody staining overlaps with antibody HPA031190.		  
Validated

Antibody staining overlaps with antibody HPA031189.		  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.   Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles & the Golgi apparatus.   Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles & the Golgi apparatus.  

Antibody dilution

 1:200   1:51   1:106  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031188

  

Antibody HPA031189

  

Antibody HPA031190

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA031189 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA031188 across 74 cell types.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.   Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 33 cell types		   RNA: detected in 37 tissues
Protein: detected in 43 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:1000   1:400    

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA031188

  

Antibody HPA031189

  

Antibody HPA031190

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 90.3   90.3   90.3  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA031188

  

Antibody HPA031189

  

Antibody HPA031190

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA031188

  

Antibody HPA031189

  

Antibody HPA031190

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 75   103   88  

Antigen sequence

 
TMILTLKDKGVLQEEEDVLVNVNLVDKERAEKNVELRKKKPDYLPYAEDE
SVDDLAQQKPRSILSKYDEELEGER
  
RRVKKIRKKEKEVVVRADDLLPLGDQTQDGDFGSRLRGRGRRRVSEVEEE
KEPVPQPLPSDDTRVENMDISDEEEGGAPPPGSPQVLEEDEAELELQKQL
EKG
  
ASSTTILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSA
VYCIEDKMAIDDKYSRREEYRGFTQDFKEKDGYKPDVK
 

Matching transcripts

 SART1-001 - ENSP00000310448 [100%]
  SART1-001 - ENSP00000310448 [100%]
  SART1-001 - ENSP00000310448 [100%]
 

Other gene match

      
ANTIGEN VIEW
SART1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.