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DLEU1

ANTIBODY INFORMATION
 
 

Antibody HPA039465

  

Antibody CAB017848

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA039465   2666.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 12   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA039465

  

Antibody CAB017848

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039465

  

Antibody CAB017848

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039465

  

Antibody CAB017848

  

Standard validation

   Uncertain  

   

Figure description

   Immunohistochemical staining of human stomach shows moderate cytoplasmic and occasional nuclear positivity in glandular cells.  

Expression

   RNA: detected in 36 tissues
Protein: detected in 66 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:150  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039465

  

Antibody CAB017848

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 9   9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA039465

  

Antibody CAB017848

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA039465

  

Antibody CAB017848

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 76    

Antigen sequence

 
MRPCIWIHVHLKPPCRLVELLPFSSALQGLSHLSLGTTLPVILPERNEEQ
NLQELSHNADKYQMGDCCKEEIDDSI
  

											 
																				

																		

										
											
Matching transcripts

												
																					DLEU1-033 - ENSP00000367422 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DLEU1-033
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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