We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Antibody dilution
1:25
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA003015
Antibody CAB011676
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 63 cell types
RNA: detected in 37 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003015
Antibody CAB011676
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418252)
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
27.8, 26, 23.7
27.8, 26, 23.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003015
Antibody CAB011676
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
