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Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.
Antibody dilution
1:189
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA042121
Antibody HPA042701
Antibody HPA043056
Antibody HPA045090
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatids.
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in mature spermatids.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in cells in seminiferus ducts.
Expression
RNA: detected in 36 tissues Protein: detected in 8 cell types
RNA: detected in 36 tissues Protein: detected in 25 cell types
RNA: detected in 36 tissues Protein: detected in 9 cell types
RNA: detected in 36 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:250
1:1000
1:175
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042121
Antibody HPA042701
Antibody HPA043056
Antibody HPA045090
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
