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Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Antibody dilution
1:40
1:25
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA003817
Antibody HPA015007
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows strong nuclear(nucleolar) and cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 38 cell types
RNA: detected in 37 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:200
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003817
Antibody HPA015007
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416921)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
39.8
39.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA003817
Antibody HPA015007
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3000
ANTIGEN INFORMATION
Antibody HPA003817
Antibody HPA015007
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
151
149
Antigen sequence
MDVNSQRALLVWGIPVNCDEAEIEETLQAAMPQVSYRMLGRMFWREENAK
AALLELTGAVDYAAIPREMPGKGGVWKVLFKPPTSDAEFLERLHLFLARE
GWTVQDVARVLGFQNPTPTPGPEMPAEMLNYILDNVIQPLVESIWYKRLT
L
