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Antibody staining overlaps with antibody HPA067385.
Validated
Antibody staining overlaps with antibody HPA058210.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus.
Antibody dilution
1:189
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
WESTERN BLOT
Antibody HPA058210
Antibody HPA067385
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
261.5, 258.4
261.5, 258.4
Antibody dilution
1:630
1:670
PROTEIN ARRAY
Antibody HPA058210
Antibody HPA067385
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
