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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Antibody dilution
1:200
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA035135
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts, Leydig cells were moderately stained.
Expression
RNA: detected in 1 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
Antibody dilution
1:500
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035135
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404199)
Target mass (kDa)
26.1, 22.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA035135
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
