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ADAMTSL1

ANTIBODY INFORMATION
 
 

Antibody HPA057437

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA057437  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 12  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA057437

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA057437

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 25 tissues
Protein: detected in 23 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:350  

Literature conformity

 Not consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA057437

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 193.4, 50.6, 15.9  

Antibody dilution

 1:370  

PROTEIN ARRAY
 
 

Antibody HPA057437

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4400  

ANTIGEN INFORMATION
 
 

Antibody HPA057437

  

Antigen

 Recombinant protein fragment  

Length (aa)

 126  

Antigen sequence

 
QVPTQLEDIRALLAATGPNLPSVLTSPLGTQLVLDPGNSALLGCPIKGHP
VPNITWFHGGQPIVTATGLTHHILAAGQILQVANLSGGSQGEFSCLAQNE
AGVLMQKASLVIQDYWWSVDRLATCS
 

Matching transcripts

 ADAMTSL1-011 - ENSP00000369918 [100%]
ADAMTSL1-012 - ENSP00000369921 [100%]
ADAMTSL1-014 - ENSP00000369911 [81%]
 

Other gene match

  
ANTIGEN VIEW
ADAMTSL1-011
ADAMTSL1-012
ADAMTSL1-014
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.