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IMPDH2

ANTIBODY INFORMATION
 
 

Antibody HPA001400

  

Antibody CAB020717

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA001400   3032.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

 IMPDH1 - ENSG00000106348 [80%]
    

Released in version

 2   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA001400

  

Antibody CAB020717

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001400

  

Antibody CAB020717

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol & rods & rings.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

 1:29   1:25  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001400

  

Antibody CAB020717

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells.   Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:350   1:100  

Literature conformity

 Consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001400

  

Antibody CAB020717

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 64.3, 63.3, 60.9, 60.4, 55.8, 55.3, 54.8, 52.6, 51, 31.2, 30.6   55.8, 51, 30.6  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001400

  

Antibody CAB020717

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001400

  

Antibody CAB020717

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 127    

Antigen sequence

 
GFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRD
IDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIV
NEDDELVAIIARTDLKKNRDYPLASKD
   

Matching transcripts

 IMPDH2-001 - ENSP00000321584 [100%]
IMPDH2-006 - ENSP00000393525 [100%]
IMPDH2-010 - ENSP00000403502 [100%]
   

Other gene match

 IMPDH1 - ENSG00000106348 [80%]
    
ANTIGEN VIEW
IMPDH2-001
IMPDH2-006
IMPDH2-010
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.