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PPP1R42

ANTIBODY INFORMATION
 
 

Antibody HPA024313

  

Antibody HPA027983

  

Antibody HPA028628

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA024313   HPA027983   HPA028628  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   6   6  

References

     1  

VALIDATION SUMMARY
 
 

Antibody HPA024313

  

Antibody HPA027983

  

Antibody HPA028628

  
Immunohistochemistry

Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024313

  

Antibody HPA027983

  

Antibody HPA028628

  

Standard validation

 Uncertain   Uncertain   Uncertain  

     

Figure description

 Immunohistochemical staining of human small intestine shows distinct cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach shows moderate nuclear positivity in glandular cells.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 66 cell types		   RNA: detected in 2 tissues
Protein: detected in 63 cell types		   RNA: detected in 2 tissues
Protein: detected in 43 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:10   1:800  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024313

  

Antibody HPA027983

  

Antibody HPA028628

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 35.5, 26.2   35.5, 26.2   35.5, 26.2  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA024313

  

Antibody HPA027983

  

Antibody HPA028628

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:20000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA024313

  

Antibody HPA027983

  

Antibody HPA028628

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 84   68   73  

Antigen sequence

 
HSLAKSLCILNISNNNIDDITDLELLENLNQLIAVDNQLLHVKDLEFLLN
KLMKLWKIDLNGNPVCLKPKYRDRLILVSKSLGT
  
LTHLYLQNNCISCIENLRSLKKLEKLYLGGNYIAVIEGLEGLGELRELHV
ENQRLPLGEKLLFDPRTL
  
MVRLTLDLIARNSNLKPRKEETISQCLKKITHINFSDKNIDAIEDLSLCK
NLSVLYLYDNCISQITNLNYATN
 

Matching transcripts

 PPP1R42-001 - ENSP00000315035 [100%]
PPP1R42-011 - ENSP00000429721 [98%]
  PPP1R42-001 - ENSP00000315035 [100%]
PPP1R42-011 - ENSP00000429721 [100%]
  PPP1R42-001 - ENSP00000315035 [100%]
PPP1R42-011 - ENSP00000429721 [100%]
 

Other gene match

      
ANTIGEN VIEW
PPP1R42-001
PPP1R42-011

RELEVANT PUBLICATIONS
 
 

Antibody HPA024313

  

Antibody HPA027983

  

Antibody HPA028628

  

1.

     PPP1R42, a PP1 binding protein, regulates centrosome dynamics in ARPE-19 cells
DeVaul N et al
Biol Cell 2013;105(8):359-71
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.