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ZNF454

ANTIBODY INFORMATION
 
 

Antibody HPA024556

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA024556  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 5  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA024556

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024556

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.  

Expression

 RNA: detected in 14 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:150  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024556

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 60  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA024556

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA024556

  

Antigen

 Recombinant protein fragment  

Length (aa)

 86  

Antigen sequence

 
MPASKKSTVKAEIPEEELDQWTIKERFSSSSHWKCASLLEWQCGGQEISL
QRVVLTHPNTPSQECDESGSTMSSSLHSAQSQGFQP
 

Matching transcripts

 ZNF454-001 - ENSP00000326249 [99%]
ZNF454-002 - ENSP00000430354 [99%]
 

Other gene match

  
ANTIGEN VIEW
ZNF454-001
ZNF454-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.