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CALML3

ANTIBODY INFORMATION
 
 

Antibody HPA044999

  

Antibody CAB010070

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA044999   1664.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

 CALM2 - ENSG00000143933 [88%]
CALM3 - ENSG00000160014 [88%]
CALM1 - ENSG00000198668 [88%]
    

Released in version

 12   3  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA044999

  

Antibody CAB010070

  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044999

  

Antibody CAB010070

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human vulva/anal skin shows moderate cytoplasmic, nuclear and membranous positivity in epidermal cells.   Immunohistochemical staining of human skin shows strong cytoplasmic positivity in epidermal cells.  

Expression

 RNA: detected in 9 tissues
Protein: detected in 55 cell types		   RNA: detected in 9 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:450   1:750  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044999

  

Antibody CAB010070

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 21.7, 20.8, 17, 16.9, 16.8, 12.9, 11.1   16.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA044999

  

Antibody CAB010070

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA044999

  

Antibody CAB010070

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 26    

Antigen sequence

 
AELRHVMTRLGEKLSDEEVDEMIRAA
  

											 
																				

																		

										
											
Matching transcripts

												
																					CALML3-001 - ENSP00000315299 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					CALM2 - ENSG00000143933 [88%]
CALM3 - ENSG00000160014 [88%]
CALM1 - ENSG00000198668 [88%]

											 
																						
											 
																				

																		

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