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Immunohistochemical staining of human skin shows strong cytoplasmic and nuclear positivity in stratum granulosum of epidermis.
Expression
RNA: detected in 6 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
Antibody dilution
1:8000
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040725
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413780)
Target mass (kDa)
15.9
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA040725
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
