AURKB - Antibodies - The Human Protein Atlas

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AURKB

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA037708

Antibody CAB005862

Provider

Atlas Antibodies
Sigma-Aldrich

Abcam plc

Product name

HPA037708

ab3609

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA037708

Antibody CAB005862

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA037708

Antibody CAB005862

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & midbody.

Antibody dilution

1:52

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA037708

Antibody CAB005862

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human tonsil shows strong nuclear positivity in subset of non-germinal center cells.

Expression

RNA: detected in 25 tissues
Protein: detected in 43 cell types

Retrieval

HIER pH6

Antibody dilution

1:35

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA037708

Antibody CAB005862

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

39.5, 39.3, 35.3, 26.2

39.5, 39.3, 35.3, 34.8, 28, 26.8, 26.2, 20, 7.8

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA037708

Antibody CAB005862

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA037708

Antibody CAB005862

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

WPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVME
NSSGTPDILTRHFTID

Matching transcripts

AURKB-001 - ENSP00000463999 [100%]
AURKB-005 - ENSP00000462207 [100%]
AURKB-008 - ENSP00000462930 [100%]
AURKB-002 - ENSP00000313950 [99%]

Other gene match

ANTIGEN VIEW

AURKB-001	AURKB-002	AURKB-005	AURKB-008

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.