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Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Antibody dilution
1:10
1:46
1:43
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA046346
Antibody HPA046695
Antibody HPA049923
Antibody HPA051288
Standard validation
Uncertain
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells, islets of Langerhans were moderately stained.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 9 tissues Protein: detected in 50 cell types
RNA: detected in 9 tissues Protein: detected in 73 cell types
RNA: detected in 9 tissues Protein: detected in 61 cell types
RNA: detected in 9 tissues Protein: detected in 42 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:200
1:75
1:80
1:65
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046346
Antibody HPA046695
Antibody HPA049923
Antibody HPA051288
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
118.2, 115.6, 46.9
118.2, 115.6, 46.9
118.2, 115.6, 46.9, 18.4
118.2, 115.6, 46.9, 18.4
Antibody dilution
1:250
1:230
1:110
1:220
PROTEIN ARRAY
Antibody HPA046346
Antibody HPA046695
Antibody HPA049923
Antibody HPA051288
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
