F2 - Antibodies - The Human Protein Atlas

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TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA051476

Antibody HPA054698

Antibody CAB016780

Antibody CAB018650

Provider

Atlas Antibodies
Sigma-Aldrich

BioPorto Diagnostics A/S

Santa Cruz Biotechnology

Product name

HPA051476

HPA054698

HYB 109-03

sc-33769

Host species

Rabbit

Mouse

Rabbit

Clonality

pAb

mAb

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA051476

Antibody HPA054698

Antibody CAB016780

Antibody CAB018650

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA051476

Antibody HPA054698

Antibody CAB016780

Antibody CAB018650

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA054698 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA051476 across 73 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in fraction of hepatocytes.

Immunohistochemical staining of human kidney shows distinct extracellular positivity.

Immunohistochemical staining of human vulva/anal skin shows distinct positivity in plasma.

Expression

RNA: detected in 1 tissues
Protein: detected in 50 cell types

RNA: detected in 1 tissues
Protein: detected in 38 cell types

RNA: detected in 1 tissues
Protein: detected in 53 cell types

RNA: detected in 1 tissues
Protein: detected in 28 cell types

Retrieval

HIER pH6

Antibody dilution

1:550

1:400

1:100

1:150

Literature conformity

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA051476

Antibody HPA054698

Antibody CAB016780

Antibody CAB018650

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

70, 65.4, 35.9

Antibody dilution

1:820

1:300

1:500

PROTEIN ARRAY

Antibody HPA051476

Antibody HPA054698

Antibody CAB016780

Antibody CAB018650

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6550

1:2150

ANTIGEN INFORMATION

Antibody HPA051476

Antibody HPA054698

Antibody CAB016780

Antibody CAB018650

Antigen

Recombinant protein fragment

Native protein

Recombinant protein

Length (aa)

Antigen sequence

FEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHV
NITRSGIECQLWRSRYPHKPEINSTTHPGADLQ

EGVWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSE
YQTFFNPRTFGSGEADCGLRPLFEKKSLE

Matching transcripts

F2-001 - ENSP00000308541 [100%]
F2-003 - ENSP00000387413 [100%]
F2-005 - ENSP00000433907 [100%]

F2-001 - ENSP00000308541 [100%]
F2-005 - ENSP00000433907 [100%]
F2-003 - ENSP00000387413 [82%]

Other gene match

ANTIGEN VIEW

F2-001	F2-003	F2-005

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.