Fields »
ABOUT
HELP
BLOG
SHISA2

ANTIBODY INFORMATION
 
 

Antibody HPA049752

  

Antibody HPA050172

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049752   HPA050172  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA049752

  

Antibody HPA050172

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049752

  

Antibody HPA050172

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & nuclear bodies.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.  

Antibody dilution

 1:24   1:26  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049752

  

Antibody HPA050172

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in subset of glial cells.    

Expression

 RNA: detected in 14 tissues
Protein: detected in 10 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:15    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA049752

  

Antibody HPA050172

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 31.4   31.4  

Antibody dilution

 1:120   1:130  

PROTEIN ARRAY
 
 

Antibody HPA049752

  

Antibody HPA050172

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:950   1:1050  

ANTIGEN INFORMATION
 
 

Antibody HPA049752

  

Antibody HPA050172

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 86   40  

Antigen sequence

 
PEGTMNNVYVNMPTNFSVLNCQQATQIVPHQGQYLHPPYVGYTVQHDSVP
MTAVPPFMDGLQPGYRQIQSPFPHTNSEQKMYPAVT
  
RCLRPKQDPQQSRAPGGNRLMETIPMIPSASTSRGSSSRQ
 

Matching transcripts

 SHISA2-001 - ENSP00000313079 [100%]
  SHISA2-001 - ENSP00000313079 [100%]
 

Other gene match

    
ANTIGEN VIEW
SHISA2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.