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Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & cell junctions.
Antibody dilution
1:20
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA028697
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human liver shows moderate cytoplasmic and nuclear positivity in hepatocytes.
Expression
RNA: detected in 34 tissues Protein: detected in 20 cell types
Retrieval
HIER pH6
Antibody dilution
1:15
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028697
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410940)
Target mass (kDa)
36.6
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA028697
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
