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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts along with additional cytoplasmic in Leydig cells.
Expression
RNA: detected in 2 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028424
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Target mass (kDa)
63.1
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA028424
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.