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Antibody staining overlaps with antibody HPA047988.
Validated
Antibody staining overlaps with antibody HPA013753.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:34
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA013753
Antibody HPA047988
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Expression
RNA: detected in 16 tissues Protein: detected in 80 cell types
Retrieval
HIER pH6
Antibody dilution
1:20
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA013753
Antibody HPA047988
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403647)
Target mass (kDa)
14.5
14.5
Antibody dilution
1:250
1:1250
PROTEIN ARRAY
Antibody HPA013753
Antibody HPA047988
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.