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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.
Expression
RNA: detected in 20 tissues Protein: detected in 13 cell types
RNA: detected in 20 tissues Protein: detected in 17 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:35
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014326
Antibody CAB014890
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407238)
Target mass (kDa)
9.7
9.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA014326
Antibody CAB014890
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.