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Antibody staining overlaps with antibody HPA016815.
Validated
Antibody staining overlaps with antibody HPA015551.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.
Antibody dilution
1:51
1:20
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA015551
Antibody HPA016815
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows strong nuclear positivity in tubular cells.
Expression
RNA: detected in 18 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
Antibody dilution
1:15
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015551
Antibody HPA016815
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414424)
Target mass (kDa)
81.1, 73.4
81.1, 73.4
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA015551
Antibody HPA016815
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
