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ANTIBODY INFORMATION
 
 

Antibody HPA040066

  

Antibody HPA044200

  

Antibody HPA077856

  

Antibody CAB017192

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Kazusa DNA Research Institute
  

Product name

 HPA040066   HPA044200   HPA077856   GX1255  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

        

Released in version

 13   13   16   4  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA040066

  

Antibody HPA044200

  

Antibody HPA077856

  

Antibody CAB017192

  
Immunocytochemistry

    Supported    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
    

Validated
  
Western blot

Uncertain   Uncertain     Supported  
Protein array

Approved   Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040066

  

Antibody HPA044200

  

Antibody HPA077856

  

Antibody CAB017192

  

Standard validation

     Supported    

       

Figure description

     Immunofluorescent staining of human cell line A549 shows localization to intermediate filaments.    

Antibody dilution

     1:196    

Literature conformity

     The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040066

  

Antibody HPA044200

  

Antibody HPA077856

  

Antibody CAB017192

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

    
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		      

 

  
 

      

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA044200 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA040066 across 46 cell lines.		      
 

Standard validation

 Supported   Supported     Supported  

       

Figure description

 Immunohistochemical staining of human smooth muscle shows strong positivity in smooth muscle cells.   Immunohistochemical staining of human heart muscle shows strong positivity in intercalated discs of myocytes.     Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 13 cell types		   RNA: detected in 35 tissues
Protein: detected in 14 cell types		     RNA: detected in 35 tissues
Protein: detected in 32 cell types		  

Retrieval

 HIER pH6   HIER pH6     HIER pH6  

Antibody dilution

 1:2500   1:1200     1:300  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040066

  

Antibody HPA044200

  

Antibody HPA077856

  

Antibody CAB017192

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		     Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

       Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 172.9, 140.2, 107.1   172.9, 140.2, 107.1     172.9, 140.2, 107.1, 39.1  

Antibody dilution

 1:250   1:250     1:500  

PROTEIN ARRAY
 
 

Antibody HPA040066

  

Antibody HPA044200

  

Antibody HPA077856

  

Antibody CAB017192

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000   1:6000   1:18000    

ANTIGEN INFORMATION
 
 

Antibody HPA040066

  

Antibody HPA044200

  

Antibody HPA077856

  

Antibody CAB017192

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 94   81   80    

Antigen sequence

 
FLGSGYSSSATTQQENSYGKAVSSQTNVRTFSPTYGLLRNTEAQVKTFPD
RPKAGDTREVPVYIGEDSTIARESYRDRRDKVAAGASESTRSNE
  
MDVSNVEAIRSRTQEAGALGVSDRGSWRDADSRNDQAVGVSFKASAGEGD
QAHREQGKEQAMFDKKVQLQRMVDQRSVISD
  
GKVGDYFATEESVGTQTSVRQLQLGPKEGFSGQIQFTAPLSDKVELGVIG
DSVHMEGLPGSSTSIRHISIGPQRHQTTQQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					SYNM-001 - ENSP00000336775 [100%] 
SYNM-002 - ENSP00000472953 [100%] 
SYNM-003 - ENSP00000453040 [100%] 

											 
																						SYNM-001 - ENSP00000336775 [100%] 
SYNM-002 - ENSP00000472953 [100%] 
SYNM-003 - ENSP00000453040 [100%] 

											 
																						SYNM-001 - ENSP00000336775 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SYNM-001
SYNM-002
SYNM-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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