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ANTIBODY INFORMATION
 
 

Antibody HPA003157

  

Antibody CAB003678

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA003157   H00000633-M01  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 2   2  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA003157

  

Antibody CAB003678

  
Immunocytochemistry

Approved    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003157

  

Antibody CAB003678

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum & the Golgi apparatus.    

Antibody dilution

 1:10    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003157

  

Antibody CAB003678

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 41 cell types		   RNA: detected in 36 tissues
Protein: detected in 13 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:350  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003157

  

Antibody CAB003678

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400642)		   Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 41.7, 26.7   41.7, 26.7  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003157

  

Antibody CAB003678

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA003157

  

Antibody CAB003678

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 140    

Antigen sequence

 
RGFWDFTLDDGPFMMNDEEASGADTSGVLDPDSVTPTYSAMCPFGCHCHL
RVVQCSDLGLKSVPKEISPDTTLLDLQNNDISELRKDDFKGLQHLYALVL
VNNKISKIHEKAFSPLRKLQKLYISKNHLVEIPPNLPSSL
  

											 
																				

																		

										
											
Matching transcripts

												
																					BGN-001 - ENSP00000327336 [100%] 
BGN-002 - ENSP00000402525 [89%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
BGN-001
BGN-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA003157

												
										 
																				
											
											
											
Antibody CAB003678

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Differential regulation of extracellular matrix protein expression in carcinoma-associated fibroblasts by TGF-β1 regulates cancer cell spreading but not adhesion

	Van Bockstal M et al

	Oncoscience 2014;1(10):634-48

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.