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Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Antibody dilution
1:200
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA045866
Standard validation
Approved
Figure description
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045866
Standard validation
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415436)
Target mass (kDa)
7.7
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA045866
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
