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COL18A1

ANTIBODY INFORMATION
 
 

Antibody HPA011025

  

Antibody HPA036104

  

Antibody CAB001961

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA011025   HPA036104   05-579  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 3   13   1  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA011025

  

Antibody HPA036104

  

Antibody CAB001961

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Approved     Approved  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA011025

  

Antibody HPA036104

  

Antibody CAB001961

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.    

Antibody dilution

   1:200    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA011025

  

Antibody HPA036104

  

Antibody CAB001961

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.     Immunohistochemical staining of human nasopharynx shows moderate nuclear positivity in respiratory epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 62 cell types		     RNA: detected in 37 tissues
Protein: detected in 24 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:125     1:100  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA011025

  

Antibody HPA036104

  

Antibody CAB001961

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 178.2, 154, 135.8, 70.7   178.2, 154, 135.8, 70.7, 30.9   178.2, 154, 135.8, 70.7, 30.9  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA011025

  

Antibody HPA036104

  

Antibody CAB001961

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA011025

  

Antibody HPA036104

  

Antibody CAB001961

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 105   72    

Antigen sequence

 
KDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSD
PNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCI
ENSFM
  
VRLWATRQAMLGQVHEVPEGWLIFVAEQEELYVRVQNGFRKVQLEARTPL
PRGTDNEVAALQPPVVQLHDSN
  

											 
																				

																		

										
											
Matching transcripts

												
																					COL18A1-001 - ENSP00000347665 [100%] 
COL18A1-002 - ENSP00000383191 [100%] 
COL18A1-003 - ENSP00000339118 [100%] 
COL18A1-201 - ENSP00000352798 [100%] 

											 
																						COL18A1-001 - ENSP00000347665 [100%] 
COL18A1-002 - ENSP00000383191 [100%] 
COL18A1-003 - ENSP00000339118 [100%] 
COL18A1-201 - ENSP00000352798 [100%] 
COL18A1-005 - ENSP00000415692 [85%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
COL18A1-001
COL18A1-002
COL18A1-003
COL18A1-005
COL18A1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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