CNOT10 - Antibodies - The Human Protein Atlas

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CNOT10

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA035614

Antibody HPA041450

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA035614

HPA041450

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA035614

Antibody HPA041450

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA035614

Antibody HPA041450

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, cytosol & centrosome.

Antibody dilution

1:111

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA035614

Antibody HPA041450

Standard validation

Approved

Figure description

Immunohistochemical staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 71 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA035614

Antibody HPA041450

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

89.1, 82.3, 79.4, 24.7

89.1, 82.3, 79.4

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA035614

Antibody HPA041450

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA035614

Antibody HPA041450

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

DNLMALNHADKLLQQPKLSGSLKFLGHLYAAEALISLDRISDAITHLNPE
NVTDVSLGISSNEQDQGSDKGENEAMESSGKRAPQCYPSSVNSARTV

PLAAFECLIEAVQVYHANPRLWLRLAECCIAANKGTSEQETKGLPSKKGI
VQSIVGQGYHRKIVLASQSIQNTVYNDGQSSAIPV

Matching transcripts

CNOT10-001 - ENSP00000330060 [100%]
CNOT10-003 - ENSP00000329376 [100%]
CNOT10-201 - ENSP00000399862 [100%]
CNOT10-012 - ENSP00000395385 [87%]

CNOT10-001 - ENSP00000330060 [100%]
CNOT10-003 - ENSP00000329376 [100%]
CNOT10-201 - ENSP00000399862 [100%]

Other gene match

ANTIGEN VIEW

CNOT10-001	CNOT10-003	CNOT10-012	CNOT10-201

RELEVANT PUBLICATIONS

Antibody HPA035614

Antibody HPA041450

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.