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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Antibody dilution
1:22
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA005792
Standard validation
Supported
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.
Expression
RNA: detected in 26 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
Antibody dilution
1:750
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Standard validation
Supported
Figure description
Nuclear and cytoplasmic staining in subsets of neurons, most abundant in Purkinje cells and cell in the CA2 pyramidal layer of the hippocampus.
WESTERN BLOT
Antibody HPA005792
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416805)
Target mass (kDa)
6.8
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA005792
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
ANTIGEN INFORMATION
Antibody HPA005792
Antigen
Recombinant protein fragment
Length (aa)
52
Antigen sequence
GAGATNGKDKTSGENDGQKKVQEEFDIDMDAPETERAAVAIQSQFRKFQK
KK
