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Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.
Antibody dilution
1:5
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA023861
Antibody HPA026536
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human kidney shows distinct cytoplasmic positivity in renal tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 18 cell types
RNA: detected in 37 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:300
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023861
Antibody HPA026536
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423254)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423254)
Target mass (kDa)
34.5, 34, 22.7, 20.6
34.5, 34, 22.7
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA023861
Antibody HPA026536
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
