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EIF3C

ANTIBODY INFORMATION
 
 

Antibody HPA047097

  

Antibody HPA049495

  

Antibody HPA050112

  

Antibody CAB034045

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA047097   HPA049495   HPA050112   sc-74507  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 EIF3CL - ENSG00000205609 [100%]
   EIF3CL - ENSG00000205609 [100%]
   EIF3CL - ENSG00000205609 [100%]
    

Released in version

 13   13   13   7  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA047097

  

Antibody HPA049495

  

Antibody HPA050112

  

Antibody CAB034045

  
Immunocytochemistry

Approved   Approved     Approved  
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047097

  

Antibody HPA049495

  

Antibody HPA050112

  

Antibody CAB034045

  

Standard validation

 Approved   Approved     Approved  

       

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.   Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.     Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

 1:24   1:22     1:2000  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047097

  

Antibody HPA049495

  

Antibody HPA050112

  

Antibody CAB034045

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 49 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:30   1:25   1:600   1:2000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047097

  

Antibody HPA049495

  

Antibody HPA050112

  

Antibody CAB034045

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 105.5, 105.3, 104.1   105.5, 105.3, 104.1   105.5, 105.3, 104.1   105.3, 104.1, 17.1, 15.3, 9.5  

Antibody dilution

 1:120   1:110   1:880   1:500  

PROTEIN ARRAY
 
 

Antibody HPA047097

  

Antibody HPA049495

  

Antibody HPA050112

  

Antibody CAB034045

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:950   1:900   1:7050    

ANTIGEN INFORMATION
 
 

Antibody HPA047097

  

Antibody HPA049495

  

Antibody HPA050112

  

Antibody CAB034045

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 70   100   92    

Antigen sequence

 
YYKFDYKAHQRQLTPPEGSSKSEQDQAENEGEDSAVLMERLCKYIYAKDR
TDRIRTCAILCHIYHHALHS
  
EEEEDNEGGEWERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQAR
GKKGTDRAAQIELLQLLVQIAAENNLGEGVIVKIKFNIIASLYDYNPNLA
  
NELMDILFANPNIFVGENILEESENLHNADQPLRVRGCILTLVERMDEEF
TKIMQNTDPHSQEYVEHLKDEAQVCAIIERVQRYLEEKGTTE
  

											 
																				

																		

										
											
Matching transcripts

												
																					EIF3C-001 - ENSP00000332604 [100%] 
EIF3C-007 - ENSP00000456416 [100%] 
EIF3C-010 - ENSP00000457418 [100%] 
EIF3C-015 - ENSP00000457963 [100%] 
EIF3C-201 - ENSP00000378953 [100%] 

											 
																						EIF3C-001 - ENSP00000332604 [100%] 
EIF3C-007 - ENSP00000456416 [100%] 
EIF3C-010 - ENSP00000457418 [100%] 
EIF3C-015 - ENSP00000457963 [100%] 
EIF3C-201 - ENSP00000378953 [100%] 

											 
																						EIF3C-001 - ENSP00000332604 [100%] 
EIF3C-007 - ENSP00000456416 [100%] 
EIF3C-010 - ENSP00000457418 [100%] 
EIF3C-015 - ENSP00000457963 [100%] 
EIF3C-201 - ENSP00000378953 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					EIF3CL - ENSG00000205609 [100%]

											 
																						EIF3CL - ENSG00000205609 [100%]

											 
																						EIF3CL - ENSG00000205609 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
EIF3C-001
EIF3C-007
EIF3C-010
EIF3C-015
EIF3C-201
								

							

						

							
									

			

				
	

	

		
			
				
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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