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Method

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Expression

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Specificity

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Score

Antibodies

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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA047097

 

Antibody HPA049495

 

Antibody HPA050112

 

Antibody CAB034045

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
  Santa Cruz Biotechnology
 

Product name

HPA047097   HPA049495   HPA050112   sc-74507  

Host species

Rabbit   Rabbit   Rabbit   Mouse  

Clonality

pAb   pAb   pAb   mAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

EIF3CL - ENSG00000205609 [100%]
  EIF3CL - ENSG00000205609 [100%]
  EIF3CL - ENSG00000205609 [100%]
   

Released in version

13   13   13   7  

References

       

VALIDATION SUMMARY

 
 

Antibody HPA047097

 

Antibody HPA049495

 

Antibody HPA050112

 

Antibody CAB034045

 
Immunocytochemistry

Approved   Approved     Approved  
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Approved   Supported    

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA047097

 

Antibody HPA049495

 

Antibody HPA050112

 

Antibody CAB034045

 

Standard validation

Approved   Approved     Approved  

       

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.   Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.     Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

1:24   1:22     1:2000  

Literature conformity

The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA047097

 

Antibody HPA049495

 

Antibody HPA050112

 

Antibody CAB034045

 

Standard validation

Supported   Supported   Supported   Supported  

       

Figure description

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.  

Expression

RNA: detected in 37 tissues
Protein: detected in 76 cell types
  RNA: detected in 37 tissues
Protein: detected in 67 cell types
  RNA: detected in 37 tissues
Protein: detected in 74 cell types
  RNA: detected in 37 tissues
Protein: detected in 49 cell types
 

Retrieval

HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

1:30   1:25   1:600   1:2000  

Literature conformity

Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA047097

 

Antibody HPA049495

 

Antibody HPA050112

 

Antibody CAB034045

 

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.
  Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.
  Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.
  Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.
 

       

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

105.5, 105.3, 104.1   105.5, 105.3, 104.1   105.5, 105.3, 104.1   105.3, 104.1, 17.1, 15.3, 9.5  

Antibody dilution

1:120   1:110   1:880   1:500  

PROTEIN ARRAY

 
 

Antibody HPA047097

 

Antibody HPA049495

 

Antibody HPA050112

 

Antibody CAB034045

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
  Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
  Supported

Pass with single peak corresponding to interaction only with its own antigen.
   

       

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

1:950   1:900   1:7050    

ANTIGEN INFORMATION

 
 

Antibody HPA047097

 

Antibody HPA049495

 

Antibody HPA050112

 

Antibody CAB034045

 

Antigen

Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

70   100   92    

Antigen sequence

YYKFDYKAHQRQLTPPEGSSKSEQDQAENEGEDSAVLMERLCKYIYAKDR
TDRIRTCAILCHIYHHALHS
 
EEEEDNEGGEWERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQAR
GKKGTDRAAQIELLQLLVQIAAENNLGEGVIVKIKFNIIASLYDYNPNLA
 
NELMDILFANPNIFVGENILEESENLHNADQPLRVRGCILTLVERMDEEF
TKIMQNTDPHSQEYVEHLKDEAQVCAIIERVQRYLEEKGTTE
 
 

Matching transcripts

EIF3C-001 - ENSP00000332604 [100%]
EIF3C-007 - ENSP00000456416 [100%]
EIF3C-010 - ENSP00000457418 [100%]
EIF3C-015 - ENSP00000457963 [100%]
EIF3C-201 - ENSP00000378953 [100%]
  EIF3C-001 - ENSP00000332604 [100%]
EIF3C-007 - ENSP00000456416 [100%]
EIF3C-010 - ENSP00000457418 [100%]
EIF3C-015 - ENSP00000457963 [100%]
EIF3C-201 - ENSP00000378953 [100%]
  EIF3C-001 - ENSP00000332604 [100%]
EIF3C-007 - ENSP00000456416 [100%]
EIF3C-010 - ENSP00000457418 [100%]
EIF3C-015 - ENSP00000457963 [100%]
EIF3C-201 - ENSP00000378953 [100%]
   

Other gene match

EIF3CL - ENSG00000205609 [100%]
  EIF3CL - ENSG00000205609 [100%]
  EIF3CL - ENSG00000205609 [100%]
   
ANTIGEN VIEW
EIF3C-001
EIF3C-007
EIF3C-010
EIF3C-015
EIF3C-201
 

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The Human Protein Atlas project is funded
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