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NOMO2

ANTIBODY INFORMATION
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046697   HPA061174  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 NOMO3 - ENSG00000103226 [100%]
NOMO1 - ENSG00000103512 [100%]
   NOMO3 - ENSG00000103226 [100%]
NOMO1 - ENSG00000103512 [100%]
  

Released in version

 13   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA046697

  

Antibody HPA061174

  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 68 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:160   1:200  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 139.5, 139.4, 134.3, 134.2, 134.1, 122.1   139.5, 139.4, 134.3, 134.2, 134.1, 122.1, 31.5  

Antibody dilution

 1:260   1:170  

PROTEIN ARRAY
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2050   1:2050  

ANTIGEN INFORMATION
 
 

Antibody HPA046697

  

Antibody HPA061174

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 94   86  

Antigen sequence

 
GEKITVTPSSKELLFYPPSMEAVVSGESCPGKLIEIHGKAGLFLEGQIHP
ELEGVEIVISEKGASSPLITVFTDDKGAYSVGPLHSDLEYTVTS
  
FRFEPSSQMIEVQEGQNLKITITGYRTAYSCYGTVSSLNGEPEQGVAMEA
VGQNDCSIYGEDTVTDEEGKFRLRGLLPGCVYHVQL
 

Matching transcripts

 NOMO2-001 - ENSP00000331851 [100%]
NOMO2-002 - ENSP00000370883 [100%]
NOMO2-201 - ENSP00000439970 [100%]
NOMO2-203 - ENSP00000477502 [100%]
NOMO2-204 - ENSP00000478653 [100%]
  NOMO2-001 - ENSP00000331851 [100%]
NOMO2-002 - ENSP00000370883 [100%]
NOMO2-201 - ENSP00000439970 [100%]
NOMO2-203 - ENSP00000477502 [100%]
NOMO2-204 - ENSP00000478653 [100%]
NOMO2-202 - ENSP00000477774 [91%]
 

Other gene match

 NOMO3 - ENSG00000103226 [100%]
NOMO1 - ENSG00000103512 [100%]
   NOMO3 - ENSG00000103226 [100%]
NOMO1 - ENSG00000103512 [100%]
  
ANTIGEN VIEW
NOMO2-001
NOMO2-002
NOMO2-201
NOMO2-202
NOMO2-203
NOMO2-204
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.