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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & the Golgi apparatus.
Antibody dilution
1:68
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA039049
Antibody HPA039050
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human soft tissue shows positivity in plasma.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 56 cell types
RNA: detected in 35 tissues Protein: detected in 65 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:125
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039049
Antibody HPA039050
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
54.2, 47, 43.4, 39.3, 39, 33.6, 24.3, 11.7
54.2, 47, 33.6, 24.3, 17.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA039049
Antibody HPA039050
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
