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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:154
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA063963
Antibody HPA066697
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human lateral ventricle shows moderate cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
Antibody dilution
1:1000
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA063963
Antibody HPA066697
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
92.6, 88.7
92.6, 88.7
Antibody dilution
1:430
1:520
PROTEIN ARRAY
Antibody HPA063963
Antibody HPA066697
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:5200
1:6200
ANTIGEN INFORMATION
Antibody HPA063963
Antibody HPA066697
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
36
Antigen sequence
DTSNIITVRVADGQTVQGEVWKTTPYQVAAEISQELAESTVIAKVNGELW
D
