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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA020111 across 46 cell lines. Pearson correlation with HPA004145 across 45 cell lines. Pearson correlation with HPA002940 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA020111 across 45 cell lines. Pearson correlation with HPA004145 across 45 cell lines. Pearson correlation with HPA000878 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA020111 across 45 cell lines. Pearson correlation with HPA002940 across 45 cell lines. Pearson correlation with HPA000878 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA004145 across 45 cell lines. Pearson correlation with HPA002940 across 45 cell lines. Pearson correlation with HPA000878 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA020111 across 70 cell types. Spearman correlation with HPA004145 across 68 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA020111 across 70 cell types. Spearman correlation with HPA002940 across 68 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA004145 across 70 cell types. Spearman correlation with HPA002940 across 70 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in epidermal cells.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in epidermal cells.
Immunohistochemical staining of human skin shows strong nuclear membrane and cytoplasmic positivity in epidermal cells.
Immunohistochemical staining of human skin shows cytoplasmic positivity in keratinocytes.
Expression
RNA: detected in 27 tissues Protein: detected in 24 cell types
RNA: detected in 27 tissues Protein: detected in 24 cell types
RNA: detected in 27 tissues Protein: detected in 73 cell types
RNA: detected in 27 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:500
1:250
1:250
1:1000
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000878
Antibody HPA002940
Antibody HPA004145
Antibody HPA020111
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Figure description
Target mass (kDa)
616.6, 85.1
616.6
616.6
616.6
Antibody dilution
1:500
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA000878
Antibody HPA002940
Antibody HPA004145
Antibody HPA020111
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
