PCYT2 - Antibodies - The Human Protein Atlas

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PCYT2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA023033

Antibody HPA023034

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA023033

HPA023034

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA023033

Antibody HPA023034

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA023033

Antibody HPA023034

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.

Antibody dilution

1:400

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA023033

Antibody HPA023034

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA023034 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA023033 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA023034 across 74 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA023033 across 74 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows strong positivity in tubular cells.

Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in tubular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 63 cell types

RNA: detected in 37 tissues
Protein: detected in 71 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

1:800

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA023033

Antibody HPA023034

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

45.6, 43.8, 41.4, 40.5, 35.4, 35.2

45.6, 43.8, 40.5, 35.2, 23.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA023033

Antibody HPA023034

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA023033

Antibody HPA023034

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

LDKYNCDFCVHGNDITLTVDGRDTYEEVKQAGRYRECKRTQGVSTTDLVG
RMLLVTKAHHSSQEMSSEYREYADSFGKCPG

TAELLSHFKVDLVCHGKTEIIPDRDGSDPYQEPKRRGIFRQIDSGSNLTT
DLIVQRIITNRLEYEARNQKKEAKELAFLEAARQQAAQPLGERDGDF

Matching transcripts

PCYT2-001 - ENSP00000439245 [100%]
PCYT2-002 - ENSP00000459044 [100%]
PCYT2-005 - ENSP00000458330 [100%]
PCYT2-006 - ENSP00000331719 [100%]
PCYT2-007 - ENSP00000461190 [100%]
PCYT2-003 - ENSP00000442050 [98%]
PCYT2-009 - ENSP00000458931 [84%]

PCYT2-001 - ENSP00000439245 [100%]
PCYT2-003 - ENSP00000442050 [100%]
PCYT2-005 - ENSP00000458330 [100%]
PCYT2-006 - ENSP00000331719 [100%]
PCYT2-007 - ENSP00000461190 [100%]
PCYT2-014 - ENSP00000460767 [100%]

Other gene match

ANTIGEN VIEW

PCYT2-001	PCYT2-002	PCYT2-003	PCYT2-005	PCYT2-006	PCYT2-007	PCYT2-009	PCYT2-014

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.