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Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in Leydig cells.
Expression
RNA: detected in 1 tissues Protein: detected in 24 cell types
RNA: detected in 1 tissues Protein: detected in 25 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:300
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028003
Antibody HPA055809
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405225)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405225)
Target mass (kDa)
35.4
35.4
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA028003
Antibody HPA055809
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
