ATP4B - Antibodies - The Human Protein Atlas

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ATP4B

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA045400

Antibody HPA052649

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA045400

HPA052649

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA045400

Antibody HPA052649

Immunohistochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA045400

Antibody HPA052649

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA052649 across 45 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA045400 across 45 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA052649 across 71 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA045400 across 71 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in subset of glandular cells.

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in subset of glandular cells.

Expression

RNA: detected in 2 tissues
Protein: detected in 2 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:25

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA045400

Antibody HPA052649

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.

Figure description

Target mass (kDa)

33.4

Antibody dilution

1:250

1:80

PROTEIN ARRAY

Antibody HPA045400

Antibody HPA052649

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA045400

Antibody HPA052649

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

TPDYQDQLRSPGVTLRPDVYGEKGLEIVYNVSDNRTWADLTQTLHAFLAG
YSPAAQEDSINCTSEQYFFQESFRAPNHTKFSCKFTADML

LQNCSGLADPNFGFEEGKPCFIIKMNRIVKFLPSNGSAPRVDCAFLDQPR
ELGQPLQVKYYPPNGTFSLHYFP

Matching transcripts

ATP4B-001 - ENSP00000334216 [100%]

Other gene match

ANTIGEN VIEW

ATP4B-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.