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Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
Antibody dilution
1:25
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA054111
Antibody HPA076894
Standard validation
Approved
Figure description
Immunohistochemical staining of human tonsil shows strong nuclear positivity in a subset of non-germinal center cells. Germinal center cells exhibited moderate cytoplasmic and nuclear staining in a fraction of cells.
Expression
RNA: detected in 36 tissues Protein: detected in 30 cell types
Retrieval
HIER pH6
Antibody dilution
1:500
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054111
Antibody HPA076894
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Target mass (kDa)
48.2
Antibody dilution
1:310
PROTEIN ARRAY
Antibody HPA054111
Antibody HPA076894
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
