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Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.
Expression
RNA: detected in 34 tissues Protein: detected in 14 cell types
RNA: detected in 34 tissues Protein: detected in 51 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:35
1:225
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA053956
Antibody HPA055430
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
73.1, 73
73.1, 73
Antibody dilution
1:110
1:230
PROTEIN ARRAY
Antibody HPA053956
Antibody HPA055430
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
