KPNA4 - Antibodies - The Human Protein Atlas

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KPNA4

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA043154

Antibody HPA045500

Antibody CAB034336

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA043154

HPA045500

sc-101547

Host species

Rabbit

Rat

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA043154

Antibody HPA045500

Antibody CAB034336

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA043154

Antibody HPA045500

Antibody CAB034336

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Relative fluorescence intensity (RFI)

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:30

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nuclear membrane.

Antibody dilution

1:30

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA043154

Antibody HPA045500

Antibody CAB034336

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA045500 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA043154 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA045500 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA043154 across 73 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.

Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells of seminiferous ducts.

Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 67 cell types

RNA: detected in 37 tissues
Protein: detected in 72 cell types

RNA: detected in 37 tissues
Protein: detected in 54 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:35

1:100

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA043154

Antibody HPA045500

Antibody CAB034336

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

57.9

Loading control

Total protein image

Antibody dilution

1:66

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

57.9

57.9, 18.9

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA043154

Antibody HPA045500

Antibody CAB034336

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3000

ANTIGEN INFORMATION

Antibody HPA043154

Antibody HPA045500

Antibody CAB034336

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

DPSLVPEAIQGGTFGFNSSANVPTEGFQF

KRRNVPHEDICEDSDIDGDYRVQNTSLEAIVQ

Matching transcripts

KPNA4-001 - ENSP00000334373 [100%]

Other gene match

ANTIGEN VIEW

KPNA4-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.