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Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Antibody dilution
1:71
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA054567
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human salivary gland shows strong positivity in myoepithelium.
Expression
RNA: detected in 35 tissues Protein: detected in 28 cell types
Retrieval
HIER pH6
Antibody dilution
1:450
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054567
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
118.8
Antibody dilution
1:350
PROTEIN ARRAY
Antibody HPA054567
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
