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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Expression
RNA: detected in 0 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
Antibody dilution
1:225
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA044739
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403632)
Target mass (kDa)
43.7
Antibody dilution
1:125
PROTEIN ARRAY
Antibody HPA044739
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
