TEAD1 - Antibodies - The Human Protein Atlas

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TEAD1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA057339

Antibody HPA062471

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA057339

HPA062471

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA057339

Antibody HPA062471

Immunocytochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA057339

Antibody HPA062471

Standard validation

Supported

Approved

Figure description

Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line SiHa shows localization to nucleus & cytosol.

Antibody dilution

1:30

1:110

Literature conformity

The subcellular location is supported by literature.

The subcellular location is partly supported by literature or no literature is available.

WESTERN BLOT

Antibody HPA057339

Antibody HPA062471

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

47.9, 41.1, 40.8, 40, 37.1

47.9, 41.1, 16.6

Antibody dilution

1:100

1:370

PROTEIN ARRAY

Antibody HPA057339

Antibody HPA062471

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1050

1:4400

ANTIGEN INFORMATION

Antibody HPA057339

Antibody HPA062471

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

VSATAIHNKLGLPGIPRPTFPGAPGFWPGMIQTGQPGSSQDVKPFVQQAY
PIQPAVTAPIPGFEPASA

IEPSSWSGSESPAENMERMSDSADKPIDND

Matching transcripts

TEAD1-001 - ENSP00000435233 [100%]
TEAD1-002 - ENSP00000435977 [100%]
TEAD1-007 - ENSP00000435393 [100%]
TEAD1-201 - ENSP00000334754 [100%]
TEAD1-202 - ENSP00000354588 [100%]

TEAD1-001 - ENSP00000435233 [100%]
TEAD1-002 - ENSP00000435977 [100%]
TEAD1-006 - ENSP00000432587 [100%]

Other gene match

ANTIGEN VIEW

TEAD1-001	TEAD1-002	TEAD1-006	TEAD1-007	TEAD1-201	TEAD1-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.