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ANTIBODY INFORMATION
 
 

Antibody HPA055376

  

Antibody HPA069552

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA055376   HPA069552  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA055376

  

Antibody HPA069552

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA055376

  

Antibody HPA069552

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line AF22 shows localization to the Golgi apparatus.    

Antibody dilution

 1:21    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA055376

  

Antibody HPA069552

  

Standard validation

   Supported  

   

Figure description

   Immunohistochemical staining of human soft tissue shows moderate positivity in peripheral nerves.  

Expression

   RNA: detected in 4 tissues
Protein: detected in 10 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:1350  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA055376

  

Antibody HPA069552

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 158.5, 155.8   158.5, 155.8, 117.9  

Antibody dilution

 1:80   1:300  

PROTEIN ARRAY
 
 

Antibody HPA055376

  

Antibody HPA069552

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:850   1:3550  

ANTIGEN INFORMATION
 
 

Antibody HPA055376

  

Antibody HPA069552

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 90   98  

Antigen sequence

 
GDIGIYRCSARNPASSRTGNEAEVRILSDPGLHRQLYFLQRPSNVVAIEG
KDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSN
  
ACVRPTHPLRSFANPLLPPPMSAIEPKVPYTPLLSQPGPTLPKTHVKTAS
LGLAGKARSPLLPVSVPTAPEVSEESHKPTEDSANVYEQDDLSEQMAS
 

Matching transcripts

 DCC-001 - ENSP00000389140 [100%]
DCC-201 - ENSP00000397322 [100%]
  DCC-001 - ENSP00000389140 [100%]
DCC-201 - ENSP00000397322 [100%]
DCC-011 - ENSP00000464582 [88%]
 

Other gene match

    
ANTIGEN VIEW
DCC-001
DCC-011
DCC-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.