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PRR27

ANTIBODY INFORMATION
 
 

Antibody HPA036150

  

Antibody HPA036151

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036150   HPA036151  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA036150

  

Antibody HPA036151

  
Immunohistochemistry


Validated
   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036150

  

Antibody HPA036151

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 2 cell types		   RNA: detected in 1 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:1700  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036150

  

Antibody HPA036151

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 22.7   22.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA036150

  

Antibody HPA036151

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA036150

  

Antibody HPA036151

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 88   83  

Antigen sequence

 
RRFPFIGEDDNDDGHPLHPSLNIPYGIRNLPPPLYYRPVNTVPSYPGNTY
TDTGLPSYPWILTSPGFPYVYHIRGFPLATQLNVPPLP
  
RGFPFVPPSRFFSAAAAPAAPPIAAEPAAAAPLTATPVAAEPAAGAPVAA
EPAAEAPVGAEPAAEAPVAAEPAAEAPVGVEPA
 

Matching transcripts

 PRR27-001 - ENSP00000343172 [100%]
PRR27-002 - ENSP00000426249 [100%]
  PRR27-001 - ENSP00000343172 [100%]
PRR27-002 - ENSP00000426249 [100%]
 

Other gene match

    
ANTIGEN VIEW
PRR27-001
PRR27-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.