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Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human stomach, lower shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 0 tissues Protein: detected in 13 cell types
RNA: detected in 0 tissues Protein: detected in 64 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:18
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036188
Antibody HPA036189
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY422265)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY422265)
Target mass (kDa)
35.8
35.8, 26.9, 26.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA036188
Antibody HPA036189
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
