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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nuclear membrane.
Antibody dilution
1:6
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA032154
Antibody HPA050618
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of lymphoid cells outside reaction centra and reaction center cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 19 cell types
RNA: detected in 37 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:1000
Literature conformity
Not consistent with gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA032154
Antibody HPA050618
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Figure description
Target mass (kDa)
304.8, 304.2
304.8, 304.2
Antibody dilution
1:250
1:1250
PROTEIN ARRAY
Antibody HPA032154
Antibody HPA050618
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
