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Immunofluorescent staining of human cell line U-2 OS shows localization to lipid droplets.
Antibody dilution
1:33
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA043327
Antibody HPA061740
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 3 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043327
Antibody HPA061740
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
56.4
56.4
Antibody dilution
1:250
1:110
PROTEIN ARRAY
Antibody HPA043327
Antibody HPA061740
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
