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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
Antibody dilution
1:98
1:31
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA055427
Antibody HPA066310
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
Antibody dilution
1:700
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055427
Antibody HPA066310
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
65.2, 62.7, 61.7, 54.9, 52.4
65.2, 62.7, 61.7, 54.9, 54.2, 52.4
Antibody dilution
1:490
1:100
PROTEIN ARRAY
Antibody HPA055427
Antibody HPA066310
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
