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Antibody staining overlaps with antibody HPA068918. Antibody staining overlaps with antibody HPA066936.
Validated
Antibody staining overlaps with antibody HPA068918. Antibody staining overlaps with antibody HPA066504.
Validated
Antibody staining overlaps with antibody HPA066936. Antibody staining overlaps with antibody HPA066504.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line RT4 shows localization to microtubule organizing center & vesicles.
Immunofluorescent staining of human cell line RT4 shows localization to microtubule organizing center & vesicles.
Immunofluorescent staining of human cell line RT4 shows localization to microtubule organizing center & vesicles.
Antibody dilution
1:33
1:141
1:35
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
WESTERN BLOT
Antibody HPA066504
Antibody HPA066936
Antibody HPA068918
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
92.1
92.1
92.1
Antibody dilution
1:80
1:470
1:80
PROTEIN ARRAY
Antibody HPA066504
Antibody HPA066936
Antibody HPA068918
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.